Single-Cell Culture and Analysis on a Optoelectronic Tweezers Platform [BPN733]
In contrast to bulk analysis, analyzing biological samples on a single-cell level is a powerful tool in deriving a more complete, quantitative understanding of cellular behavior. The optoelectronic tweezers (OET) platform utilizes light-generated dielectrophoretic force to manipulate micro-scale objects reconfigurably on the device surface. Consequently, OET is able to select for individual cells and manipulate them into a specific configuration where these cells are cultured and studied for an extended period of time. Compared to trap-based single-cell techniques, the OET platform provides advantages such as specific selection of an individual cell and subsequent culturing over a larger-scale area at a particular address on the surface of the device. We are looking to apply the OET platform for long- term culture of single cells and gain useful insights into topics such as rate of proliferation, differentiation, and changes in morphology and protein expression.