User: Guest |  Site Map |  My BSAC Profile
Visiting Industrial Fellows
Eric Sweet, Ph.D. 2020

Mechanical Engineering
Advisor: Prof. Lin


3D Printed Biomedical and Diagnostic Systems [BPN846]
Enzyme-linked immunosorbent assay (ELISA) kits have wide applications in medical diagnostics, quality-control
of produce, and toxicology. These kits, however, are expensive, highly complex, and require large sample volumes in
order to process data. To overcome these drawbacks, microfluidic lab-on-chip platforms have been developed to
quantify antibody-antigen binding interactions using microlitre volumes of analyte. These devices rely on standard
soft-lithography or MEMS-based manufacturing methods, which are becoming increasingly more time consuming,
labor intensive, and costly in the face of additive manufacturing processes. Additionally, current methods rely on
immunofluorescence or chromogenic detection to measure antibody-antigen binding success, but these methods are
time consuming, expensive, or subject to cross-reactivity or biofouling. We aim to develop a novel fully 3D printed
thermoelectric biosensor for antibody-antigen binding quantification as an alternative micro-ELISA diagnostic tool. In
this project, we will demonstrate that our 3D printed thermoelectric device can detect the streptavidin-biotin binding
interaction as an initial proof-of-concept. Previous thermoelectric sensors for detection of bioreactions utilize non-
biocompatible materials, which require the thermopile to be placed away from the channel, resulting in lower
sensitivity systems. Utilizing a biocompatible PEDOT:PSS-based composite as our thermoelectric material, we aim to
integrate the thermopile into the microchannel itself. Additionally, 3D printing will enable us to design 3D thermopile
geometries that cannot be otherwise achieved with traditional soft-lithography. By eliminating the need for optics or
external energy sources coupled with the use of low-cost additive manufacturing, we aim to develop a simple lab-on-
chip alternative to traditional ELISA kits.

Current Active Projects:

     Last Updated: Sun 2017-Feb-12 17:30:50

back to Researchers


  • Copyright Notification: All papers downloaded from this site are © University of California or the publisher, all rights reserved. Contact the BSAC Webmaster for permission related to copyrighted materials.
  • Links on these pages to commercial sites do not represent endorsements by UC or its affiliates.
  • Privacy Policy
  • Contact Us
  User logged in as: Guest
  User Idle since: January 20, 2018, 2:54 am