Fall 2010 IAB
September 15 to 17

Prof. Luke Lee


Molecular Diagnostics

In this talk, I will discuss molecular diagnostics and therapeutics for personalized medicine. Using new paradigms of biological inspiration and understanding of electron transfer mechanism in biological systems, we have developed quantized Plasmon Resonance Energy Transfer (PRET) nanospectroscopy for molecular imaging of living cells. For the remote optical control of gene regulation and protein expression, we have developed Oligonucleotides on a Nanoplasmonic Carrier Optical Switch (ONCOS). ONCOS allows on-demand gene silencing with nanometer-scale spatial resolution and localized temperature controls in living cells. The ONCOS and PRET are being applied for molecular/cellular diagnostics, therapeutic applications, and experimental system biology since it will provide us precise spatial and temporal information of living cellular mechanism. Bionanophotonic molecular ruler is also accomplished to measure the dynamics of DNA and protein interactions and understanding cellular dynamics. In-vivo Surface Enhanced Raman Spectroscopy (SERS) probes, in-vitro integrated nanofluidic SERS, and optofluidic ICs are developed for label-free molecular diagnostics and drug discovery. In order to accomplish physiologically relevant cell culture platforms, we have developed Biological Application Specific Integrated Circuits (BioASICs) for single cell biophysics, quantitative cell biology, and cell-based diagnostics by connecting novel microfluidics and nanofluidic circuits, which can impact on high-speed and high-content quantitative biology in new ways. We are creating a library of these “building blocks" to develop innovative single cell array, dynamic cell culture array, and cellular microprocessors, which can help us to develop an innovative personalized medicine.