Fall 2010 IAB
September 15 to 17
Case Study: Microelectrochemical Devices and Instruments for Environmental Monitoring and Clinical Diagnostics
We report our studies toward the development of manual handehled and portable fully automated instruments and reliable enzyme-based immunosensors using arrays of 50-micron diameter cavities with two or more individually addressable integrated electrodes. The microelectrodes in the microcavity exhibit lower background signals with zeptomole detection limits when the RMD is converted into a capture surface and signal generation involved the wall middle layer gold electrode as the detecting electrode with the top layer gold as the pseudoreference/auxiliary electrodes. The microcavity array has been converted into enzyme immunosensors for environmental, clinical, and homeland security applications. It has been used in the enzyme-linked immunoassay detection of Bacillus anthracis PA, malaria causing Plasmodium species, Cryptosporidium parvum in lake water and river water, Giardia lamblia, E. coli, Bacillus globigii, allergens from dust samples, and ovarian cancer protein biomarkers.