Fall 2010 IAB
September 15 to 17

Plenary 7: Frank Myers

An Integrated Microsystem for Electrophysiology-Activated Cell Sorting (EPACS)

We are developing a new technology for non-genetic, label-free cell purification based on electrophysiological response to stimulus. As many of the cell types relevant for regenerative medicine are electrically-excitable (e.g. cardiomyocytes, neurons, smooth muscle cells), this technology is well-suited for purifying cells from heterogeneous stem cell progeny in clinical applications without the risk and expense associated with labeling molecules. Our prototype cell sorter is capable of distinguishing undifferentiated human induced pluripotent stem cells (iPSC) from iPSC-derived cardiomyocytes. The system utilizes a microfluidic device with integrated electrodes for electrical stimulation and recording of extracellular field potential signals from cells in flow. This concept represents an entirely new approach to cell sorting, in which a cellís functionality is assessed rather than its expression profile or physical characteristics