BERKELEY SENSOR & ACTUATOR CENTER
UC BERKELEY UC DAVIS
User: Guest |  Site Map |  My BSAC Profile
HOME  PROJECTS  THRUSTS  PUBLICATIONS  ABOUT BSAC  DIRECTORY  ALUMNI  FOR BSAC RESEARCHERS  EVENTS CALENDAR  SECURE LOGIN
Lee
     
 Lee
 Lin
 Wu
 

BPN409: Long-term Cytotoxic Drug Assay via Single-Cell Microfluidic Array

Project ID BPN409
Website
Start Date Mon 2007-Aug-13 21:29:26
Last Updated Tue 2007-Aug-14 00:26:24
Abstract Understanding cellular dynamics of xenobiotic and ion transport are important for in-vitro drug assay since it is a critical step to estimate the performance, toxin and side effect of new drug candidates. Most current drug assays are based on the average cellular response of large cell populations. In conventional cell culture platforms the mass transport of cell cluster is affected by transport at neighboring cells. In order to study systematically xenobiotic and ion transport, isolated single cells on optofluidic multisensing platform are required. To observe cancer cells under drug exposure at a single-cell resolution while providing each cell with equivalent microenvironment provides more information-rich assay. Here we use a single cell trapping array device for drug investigations on a large amount of singles cells. The device equipped with media perfusion channel and waste exclude outlet provides best cell culture environment. The dynamic perfusion flow supplies media with/without drug to properly simulate the in-vivo drug delivery mode. Drug assays for both adherent and non-adherent cell lines were demonstrated in the device. We have evaluated dose conditions of Taxol on Hela and HL-60 cells. For Hela cells, after dose, the cells rounded up sequentially as they entering the mitotic phase and being arrested at this status. The big round cells then died with failure of division. The timing of death of each single cell was statistically analyzed. The drug resistant cells were carefully investigated. More systematic and quantitative drug assay results will be presented.
Status New
Funding Source
IAB Research Area Microfluidics
Researcher(s) Liz Wu
Advisor(s) Luke P. Lee
Detailed Information
Secure Access

Private Abstract
Research Report
Poster
Summary Slide PDF | VIDEO
Active Feedback (or Request for Response)

 

  • Copyright Notification: All papers downloaded from this site are © University of California or the publisher, all rights reserved. Contact the BSAC Webmaster for permission related to copyrighted materials.
  • Links on these pages to commercial sites do not represent endorsements by UC or its affiliates.
  • Privacy Policy
  • Contact Us

   webmaster@bsac.eecs.berkeley.edu
  User logged in as: Guest
  User Idle since: November 23, 2014, 10:46 am