BERKELEY SENSOR & ACTUATOR CENTER
UC BERKELEY UC DAVIS
User: Guest |  Site Map |  My BSAC Profile
HOME  PROJECTS  THRUSTS  PUBLICATIONS  ABOUT BSAC  DIRECTORY  ALUMNI  FOR BSAC RESEARCHERS  EVENTS CALENDAR  SECURE LOGIN
Lee
     
 Lee
 Lin
 Wu
 

BPN417: Spatially Targeted Microfluidic Stimulation for Quantitative Gap Junction Analysis

Project ID BPN417
Website
Start Date Mon 2007-Dec-10 18:02:23
Last Updated Tue 2008-Aug-19 15:29:26
Abstract Gap junctions are expressed in most mammalian cells, coupling their intracellular environments to allow direct communication of ions, metabolites, and other small molecules. Although gap junctions have been implicated in a range of diseases from neuronal degeneration to cancer, our understanding of their function is still incomplete. We present here the application of a microfluidic device for assaying gap junction communication by focusing dye molecules or other membrane diffusiblefocusing dye molecules or other membrane diffusible biomolecules over cells cultured to confluence. A laminar stream of gap junction-transmissible dye is hydrodynamically focused between two streams of buffer solution, selectively targeting a column of cells, while leaving their neighbors unexposed. Dye transfer into neighboring cells can be monitored via timelapse fluorescent microscopy, yielding dynamic information about the process of molecular transfer.
Status Continuing
Funding Source
IAB Research Area BioMEMS
Researcher(s) Sisi Chen
Advisor(s) Luke P. Lee
Detailed Information
Secure Access

Private Abstract
Research Report
Poster
Summary Slide
Active Feedback (or Request for Response)

 

  • Copyright Notification: All papers downloaded from this site are © University of California or the publisher, all rights reserved. Contact the BSAC Webmaster for permission related to copyrighted materials.
  • Links on these pages to commercial sites do not represent endorsements by UC or its affiliates.
  • Privacy Policy
  • Contact Us

   webmaster@bsac.eecs.berkeley.edu
  User logged in as: Guest
  User Idle since: July 22, 2014, 2:19 am