| Project ID |
BPN452 |
| Website |
|
| Start Date |
Sun 2008-Mar-09 13:48:37 |
| Last Updated |
Tue 2009-Feb-03 11:13:33 |
| Abstract |
We present the design, fabrication and results of microfabricated interfaces for the
patterned delivery of foreign molecules via electroporation into developing embryos. We show how
these systems can be used to ‘draw’ two-dimensional patterns of tracer molecules, DNA and mRNA into
the yolk and cells of zebrafish embryos (Danio rerio) at different stages of development. We
demonstrate the successful delivery of two-dimensional patterns of trypan blue (normal dye), texas
red (fluorescent dye), pCS2eGFP DNA and GFP-mRNA in both chorionated and dechorionated embryos.
Both DNA and mRNA were expressed in the desired patterns subsequent to delivery. |
| Status |
Completed |
| Funding Source |
|
| IAB Research Area |
Microfluidics |
| Researcher(s) |
Tushar Bansal |
| Advisor(s) |
Michel M. Maharbiz |
|
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