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BPN495: QES: Continuous Flow Cell Lysometer

Project ID BPN495
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Start Date Wed 2009-Jan-28 18:51:36
Last Updated Thu 2012-Aug-16 18:30:06
Abstract Single cell analysis is an increasingly important area of consideration. Rather than obtaining a bulk average assay result from a large number of cells, it is possible to do a statistical study on each individual cell in a population. Flow cytometry allows this methodology, but is incapable of testing for compounds inside the cells themselves. It instead relies on using surface markers. Some limited markers (calcium probes) capable of penetrating the cell wall are also available, but are limited to a few very specific tests. Our device will enable the assay of cytosolic (internal) components in individual cells in a rapid, continuous manner.
Status Continuing
Funding Source NSF
IAB Research Area Microfluidics
Researcher(s) Timothy P. Brackbill
Advisor(s) Albert P. Pisano, Frans Kuypers
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