BERKELEY SENSOR & ACTUATOR CENTER
UC BERKELEY UC DAVIS
User: Guest |  Site Map |  My BSAC Profile
HOME  PROJECTS  THRUSTS  PUBLICATIONS  ABOUT BSAC  DIRECTORY  ALUMNI  FOR BSAC RESEARCHERS  EVENTS CALENDAR  SECURE LOGIN
Lee
     
 Lee
 Lin
 Wu
 

BPN618: Reprogramming Cells on a Chip

Project ID BPN618
Website
Start Date Wed 2011-Feb-02 17:56:52
Last Updated Thu 2011-Feb-03 10:44:41
Abstract Induced Pluripotent Stem Cells (IPSCs) are stem cells that can be derived from fibroblast cells (skin). It has the potential of providing multiple tissue types in the body without any transplant rejection and also bypasses the ethical issues of embryonic stem cells. However, because parts of the cellular mechanisms for reprogramming are still unclear, IPSCs has yet to be utilized in the clinical setting since scientists cannot control the reprogramming/ differentiation process precisely. Here we present a microfluidic chip, which can be used for deciphering reprogramming dynamics. This chip can pair and fuse different cell types in the single cell level for IPSCs reprogramming studies. It has been shown the most efficient stem cell reprogramming method is via cell fusion (70%). We demonstrate the ability of single cell pairing of stem cells to fibroblasts with high efficiency. We then show the feasibility of electro-fusing these cells on chip. Furthermore, we explore the possibility of utilizing micro-structures which can keep the paired cells' nuclei separated so they do not become heterokaryons (multinuclei cells). This chip provides a valuable platform for studying the mechanism of direct cell reprogramming.
Status New
Funding Source
IAB Research Area Microfluidics
Researcher(s) Erh-Chia Yeh, Chi-Cheng Fu
Advisor(s) Luke P. Lee
Detailed Information
Secure Access

Private Abstract
Research Report
Poster
Summary Slide PDF | VIDEO
Can’t view video click here
Active Feedback (or Request for Response)

 

  • Copyright Notification: All papers downloaded from this site are © University of California or the publisher, all rights reserved. Contact the BSAC Webmaster for permission related to copyrighted materials.
  • Links on these pages to commercial sites do not represent endorsements by UC or its affiliates.
  • Privacy Policy
  • Contact Us

   webmaster@bsac.eecs.berkeley.edu
  User logged in as: Guest
  User Idle since: December 21, 2014, 4:51 am