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BPN809: Photonic Cavity Bioreactor for High-throughput Screening of Microalgae

Project ID BPN809
Website
Start Date Tue 2015-Aug-25 11:15:56
Last Updated Mon 2018-Jan-29 00:02:38
Abstract Photosynthesis of algal is considered as a sustainable, alternative and renewable solution for generating green energy. For high-productivity algaculture in diverse local environments, a high- throughput screening method is needed in selecting algal strains from naturally available or genetically engineered strains. Herein, we present an integrated plasmonic photobioreactor for rapid, high-throughput screening of microalgae. Our 3D nanoplasmonic optical cavity-based photobioreactor generates the amplification of photosynthesis in the cavity with selective wavelengths. The hemispheric plasmonic cavity promotes effective intercellular interaction and also permits effective visual examination of algal growth. Furthermore, noninvasive real-time monitoring of electrophysiological response upon photonic regulation of microalgae allows to characterize microalgal population. Using Chlamydomonas reinhardtii, we accomplish enhanced growth rate (2X) and lipid production rate (1.5X) with no distinctive lag phase. We also demonstrate that the temporal dynamics of the electrophysiological response over microalgae culture period are highly correlated with cell conditions (i.e., metabolic activity and population). By facilitating growth rates and automatic characterization of microalgal population, the integrated microalgae analysis platform (iMAP) will serve as rapid microalgae screening platforms for biofuel applications.
Status Continuing
Funding Source Foundation
IAB Research Area NanoPlasmonics, Microphotonics & Imaging
Researcher(s) Minsun Song, SoonGweon Hong
Advisor(s) Luke P. Lee
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