| Project ID |
LPL40 |
| Website |
|
| Start Date |
Thu 2005-Jan-27 16:29:45 |
| Last Updated |
Tue 2005-Aug-30 19:16:23 |
| Abstract |
Most of current clinical diagnosis is based on observation and analysis on a large number of cells, which provides averaged or integrated information about the disease state. However, many diseases start from a small number of mutated or unhealthy cells while most other cells remain normal. In order to detect these precursors as early as possible, a high throughput single cell based detection method is required for the analysis of large number of cells from tissue samples. Here we report a microfluidic device where individual cells are encapsulated in nano-liter aqueous compartments separated by non-aqueous (oil) compartments in a fast flowing micro-channel. Subsequent single cell lysis and analysis, including DNA amplification and biochemical assay, can be applied to each cell compartment on the same chip without contamination from other compartments. |
| Status |
No Activity |
| Funding Source |
Other |
| IAB Research Area |
BIOMEMS AND MICROFLUIDICS |
| Researcher(s) |
Junyu Mai, Poorya Sabounchi |
| Advisor(s) |
Luke P. Lee |
|
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